Enzyme

FACTORS AFFECTING ENZYME ACTIVITY PURDUE UNIVERSITY INSTRUMENT VAN PROJECT Factors modify Enzyme Activity INTRODUCTION The chemical chemical chemical receptions occurring in living things are controlled by enzymes. An enzyme is a protein in the kiosk which lowers the activation energy of a catalyzed reaction, thus increase the set up of the reaction. The enzyme which you will investigate in this exercise is peroxidase. Peroxidase catalyzes the reaction: 2 H2O2? 2H2 O + O2 The reaction serves to convert toxic hydrogen peroxide (H2O2), a product of metabolism, into water and oxygen. This reaction occurs spontaneously, but slowly, in the absence of peroxidase. Peroxidase simply serves to accelerate the reaction. Peroxidase is substantially extracted from turnips by homogenisation in dampen. The broken cells release peroxidase (as easy as other enzymes) into the buffer. The enzyme can then be degage from the cellular junk by filtration or centrifugation. In set to test for the posture of the enzyme, the extract is mixed with H2 O + O2and a compound normally known as Guaiacol (2-methyoxyphenol). If the enzyme is present, the peroxide is right away born-again to water and oxygen, and the oxygen reacts with the Guaiacol to produce a cook product, oxidize Guaiacol. The brown color can be deliberate in a spectrophotometer.

O2 + guaiacol ? brown color The simple assay part above will only indicate whether peroxidase is present in or absent from an extract. By modifying the basic assay, however, we can see to it more about this enzyme and about enzymatic activity in general. In the series of experime nts that follow, you will investigate the ef! fects of enzyme concentration, pH, and temperature on the rate of the peroxidase-catalyzed conversion of H2O2 to water and oxygen. INSTRUCTOR DEMONSTRATION prize the catalyzed reaction of H2O2 and O2by peroxidase enzyme. Your instructor will mix the contents of ii test tubes made up of the following contents: underground A 2 mL pH 5 buffer 1...If you penury to get a full essay, order it on our website: BestEssayCheap.com

If you want to get a full essay, visit our page: cheap essay